山羊卵巢维持基因FOXL2启动子活性及调控区域分析

doi:10.11843/j.issn.0366-6964.2015.12.005

Abstract

Abstract:

The research was designed to study the activity of goat ovarian maintenance gene FOXL2 promoter and explore the gene’s regulation mechanism.FOXL2 promoter sequence was retrieved from the NCBI database，bioinformatics software was adopted to predict its core promoter and transcription factors.PCR technology was used to clone FOXL2 gene promoter sequence and construct a series of deletion vectors，293T and A375 cells were transiently transfected，dual luciferase gene detector was used to measure the relative luciferase activity value.The results indicated that there were 2 typical CpG islands in the gene promoter region，which were located at （-920/+51(972 bp)） and （+125/+555(430 bp)） regions；the result of Kpn I and Hind Ⅲ dual enzyme digestion test suggested that the recombinant plasmid was constructed correctly；FOXL2 gene promoter fragments with different lengths were inserted into the cells.When the promoter 5′was truncated，luciferase transcriptional activity firstly increased and then decreased.The result indicate that （-934/+324） region has transcriptional activity，（-32/+324） region contain the basic elements of transcription；（-934/-456） region negatively regulates FOXL2 gene during the transcription process，（-456/-192） region is a positive regulatory region.

CLC Number:

S827
S813.3

GENG Li-ying，ZHANG Yu，LI Xiang-long，ZHOU Rong-yan，LI Lan-hui，WANG Zhi-gang. Activity and Regulation Region Analysis of Promoter of Goat FOXL2 Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(12): 2153-2160.

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https://www.xmsyxb.com/EN/Y2015/V46/I12/2153

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Activity and Regulation Region Analysis of Promoter of Goat FOXL2 Gene

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